摘要：花色苷是一种广泛存在于蓝莓等越橘类浆果中的水溶性色素。目前常采用大孔树脂将花色苷从浆果中糖、酸等物质分离出来，但是纯化效率较低。本论文采用超声波强化大孔树脂分离纯化蓝莓提取液中花色苷，研究了不同超声强度和温度对大孔树脂吸附和解吸特性的影响。研究结果表明，超声波作用后可显著提高花色苷的吸附量和解吸量，缩短吸附及解吸达到平衡的时间。进一步通过Pseudo二阶模型拟合吸附动力学曲线，表明超声波可提高速率常数。另外，通过分析吸附及解吸前后树脂的理化特性，表明超声波通过促进树脂表面的酚羟基与花色苷的羟基相互作用形成氢键，从而促进了大孔树脂与花色苷的相互作用。本研究结果对于提高花色苷的分离纯化效率及浆果副产物综合利用具有参考意义。
关键词：超声波；大孔树脂；蓝莓；花色苷 ；吸附；解吸
Ultrasound assisted adsorption and desorption of blueberry anthocyanins using macroporous resins
Abstract： Anthocyanins is a water-soluble pigment widely existed in the citrus fruits such as blueberries. Currently macroporous resins is often used to separate anthocyanins from sugar, acid and other substances in berries, but have a low purification efficiency. In this paper, ultrasound is used to enhance the separation and purification of anthocyanins on macroporous resins from the blueberry extracts, and the impact of different acoustic energy density (AED) and temperature on the adsorption and desorption features of blueberry anthocyanins on macroporous resins were studied. The results show that the adsorption and desorption capacity of anthocyanin can be significantly improved and the equilibrium time is shortened after ultrasonic effect. Furthermore, it shows that the ultrasonic can improve the rate constant through Pseudo second-order model. In addition, the analysis of physical and chemical properties of the resins before and after adsorption and desorption shows that the hydrogen bonds formed between phenol hydroxyls of resins surface and hydroxyls of anthocyanins, thus promote the interaction between macroporous resins and anthocyanins. The results of this study are useful for improving the separation and purification efficiency of anthocyanins and the comprehensive utilization of berry by-products.
Key words: Ultrasound; Macroporous resin; Blueberry; Anthocyanins; adsorption; desorption
目 录
摘要2
关键词2
Abstract2
Key words2
引言（或绪论）3
1材料与方法4
1.1材料与试剂 4
1.2仪器与设备 4
1.3大孔树脂的预处理 4
1.4蓝莓花色苷提取液的制备5
1.5超声辅助吸附过程 5
1.6震荡辅助吸附过程 5
1.7超声辅助解吸过程 5
1.8 理化分析 5
1.8.1 总花色苷含量的测定 5
1.8.2 可滴定酸和总糖含量的测定 5
1.8.3 有机酸和糖类 5
1.8.4 大孔树脂的理化特征 6
1.9 吸附动力学模拟 6
1.10 数据处理与分析 6
2结果与分析 7
2.1超声波吸附/解吸与静置吸附/解吸、震荡吸附/解吸比较 7
2.2 AED和温度对大孔树脂吸附花色苷的影响 8
2.2.1 对花色苷吸附动力学的影响 8
2.2.2 对酸类和糖类分布的影响 9
2.2.3 对树脂理化特性的影响10
2.3 AED和温度对大孔树脂解吸花色苷行为的影响12
2.3.1 对花色苷解吸动力学的影响12
2.3.2 对树脂理化特性的影响14
3 结论与展望14
致谢14
参考文献14
超声波辅助大孔树脂纯化蓝莓花色苷
蓝莓是有机酸和酚类物质，尤其是花色苷等生物活性物质的一种丰富的膳食来源。根据USDA（美国农业部）[1]的规定，它对健康有着很大益处，如抗氧化、抗癌、抗动脉粥样硬化和抗胆固醇活性[2-4]，已被FAO（联合国粮食及农业组织）列为五大健康水果之一[3]。然而，许多研究表明，蓝莓果实在脱水过程中由于糖和酸含量高而出现有严重的粘性问题，导致品质不良、产量低、能耗高[6,7]。此外，还证实了低糖酸含量的植物化学物质对肥胖病患和糖尿病患者比完整果实更有效、更健康[4]。因此，有必要生产少量或不含糖和酸的花色苷浓缩物，作为膳食补充剂或保健品。 超声波辅助大孔树脂纯化蓝莓:http://www.chuibin.com/shiping/lunwen_205983.html