耐受环境应激多样性的研究

摘要：沙门氏菌作为主要的食源性致病菌，引起的食物中毒在我国约占40%。其给人体健康和食品产业带来了极大的危害与损失。本试验研究5种沙门氏菌在三种胁迫环境---高渗透压、酸性、氧化环境下培养,菌体的耐受效应情况。结果表明，在高渗透压环境中，肠炎沙门氏菌的耐受应激效应最强，肠炎沙门氏菌标准株的耐受应激效应最弱；在酸性环境中，肠炎沙门氏菌耐受应激效应最强，鼠伤寒沙门氏菌的耐受应激效应最弱；肠炎沙门氏菌在氧化环境中短期培养后会激发其耐受应激效应，鼠伤寒沙门氏菌在氧化环境中耐受应激效应最弱；试验结果说明不同种类沙门氏菌在相同胁迫环境下，其耐受应激效应不同。

关键词：沙门氏菌；高渗透压；酸性；氧化；耐受应激

Study on tolerance to persity of environmental stress

Abstract：Salmonella is a major foodborne pathogen, causing food poisoning in China about 40%. It has brought great harm and loss to human health and food industry. In this study, 5 kinds of salmonella were studied in three kinds of stress environments - high osmotic pressure, acid and oxidizing environment, and the tolerance effect of bacteria. The results showed that, in the high osmotic pressure environment, the tolerance stress of salmonella enteritis was the strongest, and the strain of the standard strain of enteritis was the weakest. In acidic environment, salmonella enteritis had the strongest stress effect, and the resistance of salmonella typhi was the weakest. Salmonella enteritis in the oxidizing environment in the short - term culture will stimulate its resistance to stress, salmonella typhi in the oxidative environment of the most vulnerable to stress effect; The results showed that different strains of salmonella had different tolerance effects under the same stress environment.

Key words: Salmonella; High osmotic pressure; Acidity; Oxidation; Stress tolerance

摘要 1

关键词 1

Abstract 1

Key words 1

引言 1

1 材料与方法 2

1．1 试验材料 2

1．2 试剂、器材与设备 2

1．3 试验方法 3

1.3.1 沙门氏菌的高渗透压应激试验 3

1.3.2 沙门氏菌的酸性应激试验 3

1.3.3 沙门氏菌的氧化应激试验 4

2 结果与分析 5

2．1 沙门氏菌对高渗透压环境的耐受应激情况 5

2．2 沙门氏菌对酸性环境的耐受应激情况 6

2．3 沙门氏菌对氧化环境的耐受应激情况 7

3 讨论 9

3．1 沙门氏菌的高渗透压应激效应 9

3．2 沙门氏菌的酸性应激效应 9

3．3 沙门氏菌的氧化应激效应 10

3．4 总结 10

致谢 10

参考文献： 11

耐受环境应激多样性的研究

引言

沙门氏菌目前已经发现超过2 500种血清型，作为一种常见病原菌，其极易导致人类和动物患病[1]。迄今为止，沙门氏菌导致的食物中毒事件在全球范围内均有出现[2]，据悉，在中国，沙门氏菌引起的食物中毒是每年细菌性食物中毒事件中占比最大的，约达到40 %的占比[3]，导致沙门氏菌中毒的食物约有90%是肉、蛋、奶和其他畜禽产品[4-5]。在肉类和蛋类产品加工过程中，具有多个生产环节，每个环节都存在交叉污染的风险[6]，从而造成沙门氏菌的污染、传播。耐受环境应激多样性的研究:http://www.chuibin.com/shengwu/lunwen_205988.html