摘要：植物根系率先与复杂多样的土壤微生物接触。植物根际微生物利用植物根系分泌物进行生长和繁殖可能对植物产生有益或有害的影响。微生物所分泌的微生物相关分子模式能够被植物自身的模式识别受体所识别，激发植物的先天免疫反应。但是微生物相关分子模式对植物根系发育，尤其是侧根发育的影响尚不清楚。本文首先通过木霉Trichoderma guizhouense NJAU4742与模式植物拟南芥相互作用，展示其对植物生长及根系发育的影响。接着通过使用菌鞭毛蛋白flg22处理植物，展示了细菌鞭毛蛋白flg22能够抑制植物主根伸长，抑制植物侧根的发生与形成。

关键词：木霉Trichoderma guizhouense NJAU4742，细菌鞭毛蛋白flg22，拟南芥，侧根

Effects of Microbe-Associated Molecular Patterns on Plant Lateral Root Development

Abstract: Plant roots are the first organs that come in contact with perse soil microorganisms. Rhizospheric microbes, which utilize plant root exudates for growth and multiplication, are attracted to the rhizosphere, and may have either beneficial or deleterious effects on the plant. Microorganisms secrete microbe-associated molecular patterns which can be recognized by the plant's pattern recognition receptors and stimulate the plants’ innate immune response of plants. However, the effects of microbe-associated molecular patterns on plant root development, especially lateral root development, are unclear. Through the interaction between the model plant Arabidopsis thaliana and Trichoderma guizhouense NJAU4742, we show its effects on plant growth and root development. By using the bacterial flagellin flg22, we also show that bacterial flagellin flg22 can inhibit the primary root elongation and lateral root initiation and emergence.

Key words: Trichoderma guizhouense NJAU4742, bacterial flagellin flg22, Arabidopsis thaliana, lateral root

目 录

摘要 1

关键词 1

Abstract. 1

Key words 1

引言 1

1  材料与方法 3

1．1 试验材料 3

1．1．1 植物材料 3

1．1．2 真菌材料 3

1．1．3 实验试剂 3

1．2 试验方法 3

1．2．1  植物生长条件 3

1．2．2  真菌生长和植物接种实验 3

1．2．3  植物表型分析 3

1．2．4  Luciferase成像和表达分析 3

1．2．5  量化和统计分析 4

2  结果与分析 4

2．1 木霉Trichoderma guizhouense NJAU4742对植物生长的影响 4

2．1．1  木霉Trichoderma guizhouense NJAU4742对植物根系结构的影响 4

2．1．2  酸性环境胁迫下木霉对植物生长及侧根发育效果不显著 4

2．2  flg22对植物侧根发育的影响 5

2．2．1  flg22抑制植物侧根的发生与形成 5

3  讨论 MAMPs对植物侧根发育的影响:http://www.chuibin.com/shengwu/lunwen_206227.html