摘要：本文研究了不同光谱对马铃薯试管薯诱导的影响。以马铃薯早熟品种费乌瑞它试管苗为试验材料，接入含有试管薯诱导培养基的培养瓶中生长。在环境可控的LED光源组培室内，选择六个不同光谱：R(620nm)；R(660nm)；B(440nm)；B(460nm)；Y(590nm)；G(520nm)作为处理，在光密度为80umol.m-2.s-1，温度为(18±2)℃；8h/d光照，16h/d黑暗光周期条件下，培养90d后测定不同处理下试管薯的形态和生理指标并分析数据。结果表明：处理B460的每瓶薯总重(B460>B440>G520>Y590>R620>R660)、平均单薯重(B460>B440>Y590>G520>R620>R660)、大薯率都显著优于其他处理，而B440次之。说明蓝光比其他光谱更能促进马铃薯试管薯成薯，其中460nm的蓝光是最适合费乌瑞它马铃薯品种试管薯成薯的光谱。本试验结果可以为利用LED光源诱导马铃薯试管薯生产提供理论依据。
关键词：光谱；马铃薯；试管薯；成薯
Effects of Different Spectra on the Induction of Potato Microtubers
Abstract：The effects of different spectra on the potato microtubers were studied. The seedling of potato early maturing variety FAVORITA was used as a test material to grow in the culture bottle containing the induction medium of potato microtubers. In the environment controllable led light source tissue culture, six different spectra are selected as treatment: R(620nm);R(660nm);B(440nm); B(460nm);Y(590nm);G(520nm) in the conditon of light density of 80umol.m-2.s-1, temperature of (18± 2 ℃); 8h/d of illumination,16h/d of dark photoperiod, the morphological and physiological indexes of the potato microtubers with different treatments for 90days were measured and the data were analyzed after 12 weeks.The results showed that the total weight (B460>B440>G520>Y590>R620>R660), average single tuber weight (B460>B440>Y590>G520>R620>R660), large tuber rate were treated with B460 were significantly superior to other treatments,while B440 followed.The blue light is better than other spectra to promote potato of FAVORITA potato microtubers, and the treatment of the 460nm bule light is the most suitable spectral for potato microtuber. The results of this experiment can provide theoretical basis for the application of LED light source to induce potato microtubers.
Key words: spectra；potato；microtubers；potato formation
目 录
摘要1
关键词1
Abstract1
Key words1
引言1
1材料与方法2
1.1材料 2
1.1.1 供试试管苗2
1.1.2 供试LED灯2
1.1.3 试管苗继代扩繁3
1.2方法 3
1.2.1不同光谱条件下培养3
1.3形态及生理指标测定3
1.3.1形态指标测定3
1.3.2生理指标测定3
1.4数据分析4
2结果与分析4
2.1不同光谱对马铃薯试管薯形态指标影响 4
2.2不同光谱对马铃薯试管薯生理指标影响4
3讨论 6
3.1不同光谱对马铃薯试管薯成薯形态指标的影响 6
3.2不同光谱对马铃薯试管薯成薯生理指标的影响 7
4结论7
致谢7
参考文献8
不同光谱对马铃薯试管薯诱导的影响
马铃薯是全球第四大粮食作物，仅次于小麦、稻谷和玉米。中国是当下世界上马铃薯总产最多的国家[1]。目前马铃薯生产主要靠传统的块茎无性繁殖，但会存在病毒病，使毒素积累，降低马铃薯产量和品质[2]，所以传统的育种方法难以满足马铃薯生产的需求。利用茎尖组织培养技术生产的脱毒试管苗和试管薯能够有效去除病毒的积累。虽然马铃薯脱毒苗可以解决毒素积累问题，但马铃薯脱毒苗不便运输，因此脱毒试管苗生产的脱毒试管薯是脱毒种薯生产体系发展的关键[3][4]。利用马铃薯茎尖组织培养技术诱导马铃薯试管薯，可以加快良种繁育速度，助推其大规模应用于大田生产，形成实际生产力。光是植物生长过程中最重要的环境因子之一[5]。在组织培养条件下，由于培养容器透光，且试管薯的形成是在培养基上部，因此光可从光周期、光照强度、光质等方面影响试管薯的诱导。 不同光谱对马铃薯试管薯诱导的影响:http://www.chuibin.com/guanli/lunwen_205990.html