对Pb2+,Hg2+和Cu2+具有比色检测的BODIPY基荧光探针的构筑并应用于分子逻辑门

摘要：过量的铜离子、铅离子以及汞离子将会对人体或者环境造成很大的危害，因此，我们需要设计合成一种新的不需要复杂仪器设备，可通过肉眼观察进行检测的荧光探针，对这三种金属离子进行检测。然而在目前的报道中，用于金属离子检测的荧光探针一般都只能检测1-2种不同的金属离子，可以同时检测这三种金属离子的荧光探针几乎没有。因此我们设计并合成了一种BODIPY基的比色荧光传感器1，并测试其金属离子响应性。实验结果证明1对Pb2+、Hg2+和Cu2+具有较高的选择性和灵敏性。在1的乙腈溶液中加入铅离子和汞离子会导致紫外吸收峰从595 nm蓝移到572 nm处，溶液的颜色变为蓝紫色而加入铜离子后吸收峰蓝移到512 nm处，溶液的颜色都从蓝色变为紫红色。以510 nm为激发波长，当加入铅离子和汞离子之后，荧光强度分别提高了50和196倍，其最大发射峰位置从625 nm处蓝移至587 nm处。另一方面，当向溶液中加入铜离子时，其荧光也发生增强现象，最大发射峰的位置也从原本的625 nm蓝移至545 nm处。基于以上结果，通过控制化学输入信号可以得到三类逻辑门，分别为OR, INHIBT和组合逻辑门。

关键词：BODIPY；分子逻辑门； Pb2+；Hg2+；Cu2+；荧光探针

Abstract：Excessive Pb2+, Hg2+ and Cu2+ ions will cause great harm to the human body or the environment. Therefore, we need to design and synthesize a new fluorescent probe to detect these three metal ions, which and can be detected by naked eye without complex instruments. However, in the present reports, the fluorescent probes used for metal ion detection can only detect 1 or 2 different metal ions, and there is almost no fluorescent probe for simultaneous detection of the three metal ions. Therefore, a BODIPY-based colorimetric fluorescent probe 1 was synthesized. 1 displayed high selectivity and sensitivity towards Pb2+, Hg2+ and Cu2+ ions. The addition of Pb2+ and Hg2+ ions into 1 in CH3CN resulted in a significant blue-shift of the UV-Vis absorption spectra from 595 nm to 572 nm, and the addition of Cu2+ ions resulted in it blue shifted from 595 nm to 512 nm. The all colors are changed from blue to purple and fuchsia. When excited at 510 nm, the fluorescence intensity were increased by 50 and 196 times, respectively, upon addition of Pb2+ and Hg2+ ions, and a blue-shift from 625 nm to 587 nm. On the other hand, 1 with Cu2+ ions exhibited significant fluorescence enhancement with an 80 nm blue-shift. Based on these results, three logic gates (OR, INHIBT, and combinational logic gate) were obtained by controlling the chemical inputs.

Keyword: BODIPY; Molecular logic gate; Lead (II); Mercury (II); Copper (II); Fluorescent probe

摘要 I

Abstract II

1. 前言 1

1.1 荧光探针信号传递机制 1

1.1.1 光诱导电子转移机理（PET） 1

1.1.2 分子内电荷转移机理（ICT） 2

1.1.3 荧光共振能量转移机理（FRET） 2

1.2 BODIPY染料的简介 3

1.3 BODIPY类荧光探针应用 4

1.4 研究目的 5

2. 实验部分对Pb2+,Hg2+和Cu2+具有比色检测的BODIPY基荧光探针的构筑并应用于分子逻辑门:http://www.chuibin.com/cailiao/lunwen_206535.html